Identification of the peptide-binding motif recognized by the pigtail macaque class I MHC molecule Mane-A1*082:01 (Mane A*0301)
Identifieur interne : 002362 ( Main/Exploration ); précédent : 002361; suivant : 002363Identification of the peptide-binding motif recognized by the pigtail macaque class I MHC molecule Mane-A1*082:01 (Mane A*0301)
Auteurs : Carrie Moore [États-Unis] ; John Sidney [États-Unis] ; A. Michelle English [États-Unis] ; Amanda Wriston [États-Unis] ; Donald F. Hunt [États-Unis] ; Jeffrey Shabanowitz [États-Unis] ; Scott Southwood [États-Unis] ; Kate Bradley [États-Unis] ; Bernard A. P. Lafont [États-Unis] ; Bianca R. Mothé [États-Unis] ; Alessandro Sette [États-Unis]Source :
- Immunogenetics [ 0093-7711 ] ; 2012-06-01.
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Abstract
Abstract: Rhesus and pigtail macaques have proven to be valuable animal models for several important human diseases, including HIV, where they exhibit similar pathology and disease progression. Because rhesus macaques have been extensively characterized in terms of their major histocompatibility complex (MHC) class I alleles, their demand has soared, making them increasingly difficult to obtain for research purposes. This problem has been exacerbated by a continued export ban in place since 1978. Pigtail macaques represent a potential alternative animal model. However, because their MHC class I alleles have not been characterized in detail, their use has been hindered. To address this, in the present study, we have characterized the peptide binding specificity of the pigtail macaque class I allele Mane-A1*082:01 (formerly known as Mane A*0301), representative of the second most common MHC class I antigen detected across several cohorts. The motif was defined on the basis of binding studies utilizing purified MHC protein and panels of single amino acid substitution analog peptides, as well as sequences of peptide ligands eluted from Mane-A1*082:01. Based on these analyses, Mane-A1*082:01 was found to recognize a motif with H in position 2 and the aromatic residues F and Y, or the hydrophobic/aliphatic residue M, at the C-terminus. Finally, analysis of the binding of a combinatorial peptide library allowed the generation of a detailed quantitative motif that proved effective in the prediction of a set of high-affinity binders derived from chimeric SIV/HIV, an important model virus for studying HIV infection in humans.
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DOI: 10.1007/s00251-012-0600-x
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Because rhesus macaques have been extensively characterized in terms of their major histocompatibility complex (MHC) class I alleles, their demand has soared, making them increasingly difficult to obtain for research purposes. This problem has been exacerbated by a continued export ban in place since 1978. Pigtail macaques represent a potential alternative animal model. However, because their MHC class I alleles have not been characterized in detail, their use has been hindered. To address this, in the present study, we have characterized the peptide binding specificity of the pigtail macaque class I allele Mane-A1*082:01 (formerly known as Mane A*0301), representative of the second most common MHC class I antigen detected across several cohorts. The motif was defined on the basis of binding studies utilizing purified MHC protein and panels of single amino acid substitution analog peptides, as well as sequences of peptide ligands eluted from Mane-A1*082:01. Based on these analyses, Mane-A1*082:01 was found to recognize a motif with H in position 2 and the aromatic residues F and Y, or the hydrophobic/aliphatic residue M, at the C-terminus. Finally, analysis of the binding of a combinatorial peptide library allowed the generation of a detailed quantitative motif that proved effective in the prediction of a set of high-affinity binders derived from chimeric SIV/HIV, an important model virus for studying HIV infection in humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Moore, Carrie" sort="Moore, Carrie" uniqKey="Moore C" first="Carrie" last="Moore">Carrie Moore</name></noRegion><name sortKey="Bradley, Kate" sort="Bradley, Kate" uniqKey="Bradley K" first="Kate" last="Bradley">Kate Bradley</name><name sortKey="English, A Michelle" sort="English, A Michelle" uniqKey="English A" first="A. Michelle" last="English">A. Michelle English</name><name sortKey="Hunt, Donald F" sort="Hunt, Donald F" uniqKey="Hunt D" first="Donald F." last="Hunt">Donald F. Hunt</name><name sortKey="Lafont, Bernard A P" sort="Lafont, Bernard A P" uniqKey="Lafont B" first="Bernard A. P." last="Lafont">Bernard A. P. Lafont</name><name sortKey="Mothe, Bianca R" sort="Mothe, Bianca R" uniqKey="Mothe B" first="Bianca R." last="Mothé">Bianca R. Mothé</name><name sortKey="Sette, Alessandro" sort="Sette, Alessandro" uniqKey="Sette A" first="Alessandro" last="Sette">Alessandro Sette</name><name sortKey="Shabanowitz, Jeffrey" sort="Shabanowitz, Jeffrey" uniqKey="Shabanowitz J" first="Jeffrey" last="Shabanowitz">Jeffrey Shabanowitz</name><name sortKey="Sidney, John" sort="Sidney, John" uniqKey="Sidney J" first="John" last="Sidney">John Sidney</name><name sortKey="Southwood, Scott" sort="Southwood, Scott" uniqKey="Southwood S" first="Scott" last="Southwood">Scott Southwood</name><name sortKey="Wriston, Amanda" sort="Wriston, Amanda" uniqKey="Wriston A" first="Amanda" last="Wriston">Amanda Wriston</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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